PDGF-BB Protein, Human

Measured by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells, EC50 < 3.0 ng/ml.

2μg（R: reducing conditions, N: non-reducing conditions).

Primary antibody: Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb(S0B1093) at 1/5000 dilution

Lane 1: untreated NIH/3T3 whole cell lysate 20 µg

Lane 2: NIH/3T3 starve 18 hours, then treated with 50 ng/ml PDGF-BB(Competitor R) for 20 minutes whole cell lysate 20 µg

Lane 3: NIH/3T3 starve 18 hours, then treated with 50 ng/ml PDG-BB(UA040014) for 20 minutes whole cell lysate 20 µg

Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Predicted MW: 68 kDa

Observed MW: 68 kDa

Immobilized PDGF-BB Protein, Human (Cat. No. UA040014) at 1.0μg/mL (100μL/well) can bind PDGF R alpha/PDGFRA Fc Chimera Protein, Human (Cat. No. UA010718) with EC50 of 23.57-26.73ng/mL.

Immobilized PDGF-BB Protein, Human (Cat. No. UA040014) at 1.0μg/mL (100μL/well) can bind PDGF R alpha/PDGFRA His Tag Protein, Human (Cat. No. UA010720) with EC50 of 0.19-0.26μg/mL.