TNF-α Protein, Human

Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, EC50 < 40pg/ml.

Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is less than 10pg/ml.

1μg（N: non-reducing condition, R: reducing condition).

Primary antibody: Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb at 1/1000 dilution

Lane 1: HeLa treated with 100 ng/ml Calyculin A for 30 minutes, whole cell lysate 20 µg

Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes whole cell lysate 20 µg

Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Predicted MW: 60 kDa

Observed MW: 65 kDa

Immobilized TNF-α Protein, Human (Cat. No. UA040018) at 0.2μg/mL (100μL/well) can bind TNFR-1/CD120a Protein, Human (Cat. No. UA040030) with EC50 of 1.90-2.41ng/mL.

Anti-His antibody Immobilized on CM5 Chip captured TNFR-1/CD120a His Tag, Human (Cat. No. UA040028), can bind TNF-α, Human (Cat. No. UA040018) with an affinity constant of 0.23 nM as determined in SPR assay.

Anti-His antibody Immobilized on CM5 Chip captured TNFR-2/CD120b His Tag, Human (Cat. No. UA010138), can bind TNF-α, Human (Cat. No. UA040018) with an affinity constant of 0.71 nM as determined in SPR assay.