Measured by its binding ability in a functional ELISA. When Recombinant Human IL-17A Free Tag, Human is immobilized 2µg/mL (100 µl/well), Recombinant Human IL-17RA His, Human binds with an EC50 of 0.47-1.5μg/ml.
Immobilized IL-17F Protein, Human (Cat. No. UA040308) at 5μg/mL (100μL/well) can bind IL-17 RA Protein, Human (Cat. No. UA040024) with EC50 of 0.73-0.88 μg/mL.
SPR
Anti-His antibody Immobilized on CM5 Chip captured IL-17 RA Protein, Human (Cat. No. UA040024), can bind IL-17F Protein, Mouse (Cat. No. UA040476) with an affinity constant of 4.331μM as determined in SPR assay.
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