Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
The EC50 for this effect is less than 50ng/mL in the presence of 5ng/mL Recombinant Human Wnt‑3a.
Immobilized RSPO3 His Tag, Human (Cat. No. UA040025) at 2.0μg/mL (100μL/well) can bind ZNRF3 Fc Chimera Protein, Human (Cat. No. UA011119) with EC50 of 1.59-3.16 ng/mL.
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