TNFR-1/CD120a Protein, Human

Immobilized TNF-α, Human (Cat. No. UA040005) at 10μg/ml (100μl/well) can bind TNFR-1/CD120a His Tag, Human (Cat. No. UA040028) with EC50 of 0.167 μg/ml.

Measured by its ability to inhibit the TNF-α mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The EC50 for this effect is less than 2.5ng/mL in the presence of 0.25 ng/mL Recombinant Human TNF‑α.

2μg (R: reducing conditions, N: non-reducing conditions).

Immobilized TNF-α Protein, Human (Cat. No. UA040018) at 0.2μg/mL (100μL/well) can bind TNFR-1/CD120a Protein, Human (Cat. No. UA040028) with EC50 of 10.0-19.4ng/mL.

Anti-His antibody Immobilized on CM5 Chip captured TNFR-1/CD120a His Tag, Human (Cat. No. UA040028), can bind TNF-α, Human (Cat. No. UA040005) with an affinity constant of 0.106nM as determined in SPR assay.

Anti-His antibody Immobilized on CM5 Chip captured TNFR-1/CD120a His Tag, Human (Cat. No. UA040028), can bind TNF-α, Human (Cat. No. UA040018) with an affinity constant of 0.23 nM as determined in SPR assay.

Anti-His antibody Immobilized on CM5 Chip captured TNFR-1/CD120a His Tag, Human (Cat. No. UA040028), can bind TNF-α(80-235aa), Mouse (Cat. No. UA040059) with an affinity constant of 1.24 nM as determined in SPR assay.