TRAIL/Apo2L Protein, Human

生物活性

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  Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D，The EC50 for this effect is less than 0.8ng/ml.

电泳(SDS-PAGE)

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  1μg (R: reducing conditions, N: non-reducing conditions).

反相高效液相色谱(RP-HPLC)

ELISA

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  Immobilized TRAIL/Apo2L, Human (Cat. No. UA040039) at 2.0μg/mL (100μL/well) can bind TRAIL R1/TNFRSF10A Fc Chimera, Human (Cat. No. UA010630) with EC50 of 3.51-5.40 ng/mL.
  

SPR

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  Anti-His antibody Immobilized on CM5 Chip captured TNFRSF10B/TRAIL R2 His Tag, Mouse (Cat. No. UA010509), can bind TRAIL/Apo2L, Human (Cat. No. UA040039) with an affinity constant of 4.67nM as determined in SPR assay.