Biological Activity:measured in a cell proliferation assay using CTLL-2 cells. EC50<2.0ng/ml
电泳(SDS-PAGE)
2μg (R: reducing conditions)
ELISA
Immobilized human IL-2RA-Fc at 2μg/ml (50μL/well) can bind to IL-2 with a linear range of 0.17ng/ml to 40ng/ml.
Immobilized IL-2 His Tag, Human (Cat. No. UA040057) at 0.5μg/mL (100μL/well) can bind IL-2R alpha/CD25 Fc Chimera, Human (Cat. No. UA010260) with EC50 of 1.23-1.99ng/ml.
SPR
Protein A Chip captured IL-2R alpha/CD25 Fc Chimera, Human (Cat. No. UA010260), can bind IL-2 His Tag, Human (Cat. No. UA040057) with an affinity constant of 15nM as determined in SPR assay.
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