TNF-α Protein, Mouse

Measured in a cytotoxicity assay using NCTC clone 929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, EC50 < 50pg/ml.

1μg (R: reducing condition, N: non-reducing condition).

Protein A Chip captured TNFR-1/CD120a Fc Chimera, Human (Cat. No. UA040030), can bind TNF-α (80-235aa), Mouse (Cat. No. UA040059) with an affinity constant of 0.27nM as determined in SPR assay.

Anti-His antibody Immobilized on CM5 Chip captured TNFR-1/CD120a His Tag, Human (Cat. No. UA040028), can bind TNF-α(80-235aa), Mouse (Cat. No. UA040059) with an affinity constant of 1.24 nM as determined in SPR assay.