IgG1 Fc, Human ,2μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 95%.
ELISA
Immobilized Biotinylated FcγRIIa/CD32a(H167) Avi&His Tag, Human (Cat. No. UA010430) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind IgG1 Fc, Human (Cat. No. UA050001) with EC50 of 0.38-0.44μg/ml.
Immobilized Biotinylated FcγRIIA/CD32a(R167) His&Avi Tag, Human(Cat. No. UA010748) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind IgG1 Fc, Human (Cat. No. UA050001) with EC50 of 0.37-0.48μg/ml.
Immobilized Biotinylated Fc γ RIIIa/CD16a(V176) Avi&His Tag Protein, Human (Cat. No. UA010763) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind IgG1 Fc Protein, Human (Cat. No. UA050001) with EC50 of 0.46-1.14μg/ml.
SPR
Anti-His antibody Immobilized on CM5 Chip captured CD16b(NA1) His Tag, Human (Cat. No. UA020006), can bind IgG1-Fc (UA050001) with an affinity constant of 9.237 μM as determined in SPR assay (Biacore T200).
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