Immunoglobulin G (IgG) is the main antibody component in normal human serum, which is produced and secreted by B cells. It is a molecule with a molecular weight of about 150kDa. IgG contains two heavy chains (50kDa) and two light chains (25kDa), which are connected by disulfide bonds. After cleavage by pepsin, IgG is divided into F (ab) s with an antigen binding site and a highly conserved Fc fragment. The Fc fragment has a highly conserved n-glycosylation site. There are 5 subtypes of IgG in mice (IgG1, IgG2a, IgG2b, IgG2c and IgG3) and 4 subtypes in rats (IgG1, IgG2a, IgG2b and IgG2c). Some studies have found that IgG2a is superior to IgG1 in activating complements. IgG2 is mainly used to neutralize antigen or block the binding of receptor ligand. In addition, human EGFR antibody IgG2 can mediate ADCC effect through myeloid cells.

IgG2b Fc (Glu97-Lys335) Protein, Mouse

电泳(SDS-PAGE)