CD20 Flag&His Tag Protein, Human (Detergent)

2 μg (R: reducing condition, N: non-reducing condition).

2e5 of transient transfected Rituximab-CD20-scFV CAR-293 cells were stained with 0.1ug CD20 Flag&His Tag Protein, Human, (Cat. No. UA060018) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity. 2e5 of transient transfected Rituximab-CD20-scFV CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.

Immobilized CD20 Flag&His tag, Human (Cat. No. UA060018) at 5μg/mL (100μL/well) can bind S-RMab® CD20 Recombinant Rabbit mAb (SDT-R133) (Cat. No. S0B2158) with EC50 of 0.30-0.43ng/mL.