Enterokinase, Bovine

5 U/μL Enterokinase, Bovine in 20 mM Tris-HCl (pH 7.4 @ 25°C), 200 mM NaCl, 2 mM CaCl2 and 50% (v/v) glycerol

Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:
1. Combine 500 ug of sample with reaction buffer
* Recommended Reaction Buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 (pH 8.0)
2. Add 1 U of Enterokinase light chain
3. Incubate at 25°C for 16 hours

1. Enterokinase is inhibited by high salt concentrations. For optimal activity NaCl concentration should be 50 mM or less. The pH of the buffer should be between 6 and 9. The enzyme requires 2 mM Calcium for activity.