IdeS Protease

Results of 5μg rabbit IgG digestion separated under different quantity of IdeS, The reaction was incubated for8 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate).

M, marker;

lane 1 rabbit IgG;

lane 2 rabbit IgG add 5 units IdeS;

lane 3 rabbit IgG add 6.25 units IdeS;

lane 4 rabbit IgG add 8.3 units IdeS;

lane 5 rabbit IgG add 12.5 units IdeS;

lane 6 rabbit IgG add 25 units IdeS

M: marker ; Complement IdeS protease，2μg on SDS-PAGE under Non-reducing and reducing condition . The purity is greater than 95%.