1. Add
the desired amount of 5mg IgG in digestion buffer or other compatible buffer*.
2. Add
IdeZ Protease to the reaction system:
• Add 1 unit of IdeZ Protease per 1µg of IgG to be digested.
• For example, add 5µl (200 units) of reconstituted IdeZ to digest 200µg
of IgG.
3. Incubate sample at 37°C for 30–60 minutes
产品数据
生物活性
Results of 5μg rabbit IgG digestion separated under different quantity of
IdeZ protease, The reaction was incubated for 30 minutes at 37°C using the
recommended digestion buffer (50mM sodium phosphate)
M,
marker:
lane 1 rabbit IgG;
lane 2 rabbit IgG add 5 units IdeZ;
lane 3 rabbit IgG add 10 units IdeZ;
lane 4 rabbit IgG add 33units IdeZ;
lane 5 rabbit IgG add 50 units IdeZ
电泳(SDS-PAGE)
M: marker; Complement IdeZ protease, 2μg on SDS-PAGE
under Non-reducing and reducing condition. The purity is greater than 95%
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营业执照（三证合一） 
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