2. Add IdeS Protease(Tag Free) to
the reaction system:
•
Add 1 unit of IdeS Protease(Tag Free) per 1µg of IgG to be digested.
•
For example, add 5µl (200 units) of reconstituted IdeS to digest 200µg of IgG.
3. Incubate sample at 37°C for
30–60 minutes
产品数据
生物活性
The results of 5μg rabbit IgG digestion separated under different quantity of IdeS Protease(Tag Free), The reaction was incubated for 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate).
M, marker;
lane 1 rabbit IgG;
lane 2 rabbit IgG add 5 units IdeS Protease (Tag Free);
lane 3 rabbit IgG add 10units IdeS Protease (Tag Free);
lane 4 rabbit IgG add 16.7 units IdeS Protease (Tag Free);
lane 5 rabbit IgG add 50 units IdeS Protease (Tag Free);
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