IdeZ Protease (Tag Free)

20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5

1. Add the desired amount of 5mg IgG in digestion buffer or other compatible buffer*.

2. Add IdeZ Protease (Tag Free)-1 to the reaction system:

• Add 1 unit of IdeZ Protease (Tag Free)-1 per 1µg of IgG to be digested.

• For example, add 5µl (200 units) of reconstituted IdeZ to digest 200µg of IgG.

3. Incubate sample at 37°C for 30–60 minutes

Please avoid repeated freeze-thaw cycles.