Pyrophosphatase, Inorganic（无机焦磷酸酶）

生物活性

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  The 100μl Reaction system was prepared: 10μl 10*Reaction Buffer, 1μl 10mM Na4P2O7, 1μl of diluted Pyrophosphatase, Inorganic or Storage (Dilution) Buffer, as well as DEPC-treated Water, incubate at 25ºC for 10 minutes. During incubation, 5mM phosphate standard was diluted with DEPC-treated Water to 0, 2.5, 5, 10, 20, 30, 40, 50μM. Malachite Green Reagent A and Malachite Green Reagent B are mixed according to the volume ratio of 2:1 (now used), that is, the color developing agent. Add 200μl phosphate standard or sample with different concentration (100μl reaction liquid +100μl water) to each hole of the 96-well plate, add 70μl color developer to each hole, gently blow and mix, stand at room temperature for 30 minutes, and determine the absorbance of 620nm wavelength with an enzymoleter. According to the concentration of phosphate standard and the value of A620, the standard curve is drawn, and the phosphate concentration in the sample is calculated according to the standard curve.
  

电泳(SDS-PAGE)

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  1μg (R: reducing condition, N: non-reducing condition).

体积排阻色谱(SEC-HPLC)