Endo S

The experimental design added 10 U, 2 U, 1 U, 0.5 U, 0.4U and 0.25 U enzymes into the 20 ul reaction system to investigate the effect of Endo S on the digestion of human IgG.

M marker

Lan1 human IgG 1μg

Lan2 human IgG 1μg+10 U Endo S

Lan3 human IgG 1μg+2 U Endo S

Lan4 human IgG 1μg+1 U Endo S

Lan5 human IgG 1μg+0.5 U Endo S

 Lan6 human IgG 1μg+0.4 U Endo S

Lan7 human IgG 1μg+0.25 U Endo S

1μg (R: reducing condition, N: non-reducing condition).