Endo S2

生物活性

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  The experimental design added 20 U, 10 U, 5 U, 2.5 U, 1U and 0.5 U enzymes into the 20 ul reaction system to investigate the effect of Endo S2 on the digestion of human IgG.
  M marker
  Lan1 human IgG 5μg
  Lan2 human IgG 5μg+20 U Endo S2
  Lan3 human IgG 5μg+10 U Endo S2
  Lan4 human IgG 5μg+5 U Endo S2
  Lan5 human IgG 5μg+2.5 U Endo S2
  Lan6 human IgG 5μg+1 U Endo S2
  Lan7 human IgG 5μg+0.5 U Endo S2

电泳(SDS-PAGE)

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  1μg (R: reducing condition, N: non-reducing condition).
  

反相高效液相色谱(RP-HPLC)