20mM Tris-HCl 、100mM NaCl、Mannitol、Carboxypeptidase B
操作步骤
The recommended amount of Carboxypeptidase B is 1:50~1:1000 (w/w) compared with the target protein (mass ratio), the optimal pH is 7.5-9.0, and the appropriate enzyme digestion temperature is 25℃~37℃.
注意事项
1. For different protein samples, it is necessary to explore the optimal enzyme concentration and reaction time.
2. Reaction at 25℃~37℃, the reaction time can be appropriately extended when digestion at 25℃.
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