DDB1/CRBN Complex, Flag&His Tag, Human

The DDB1/CRBN Complex Flag&His Tag Protein, Human activity was detected using HTRF technology. The reaction was performed by incubating the Human GSPT1, GST tag Protein, DDB1/CRBN Complex Flag&His Tag Protein, Human and varying concentration of CC-885 and antibody at 25℃ for 120 min, then reading ratio 665/620 on BMG.

1μg (R: reducing condition, N: non-reducing condition).