M-MLV (H-) Reverse Transcriptase
M-MLV[H-] RT,M-MLV[H-] Reverse Transcriptase
- 价格: ¥240
- 规格:
- 数量:

产品介绍
- 产品介绍
- 操作详情
产品组分
Storage Solution : 100 U/ul M-MLV(H-) Reverse Transcriptase、20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA、0.01% Nonidet® P-40、50% Glycerol pH 7.5 @ 25°C
5*Reaction Buffer: 250 mM Tris-HCl、375 mM KCl、15 mM MgCl2、50 mM DTT (pH 8.3 at 25°C)操作步骤
1. Genomic DNA was removed from the extracted cell total RNA by DNase I.
2. After incubating at 37 °C for 30 minutes, add 1µl 0.5 M EDTA (to the final concentration of 5mm) and inactivate at 75°C for 10 minutes.
3. Mix RNA sample, Random Primer and 1 ul M-MLV(H-) Reverse Transcriptase in a sterile RNase-free microfuge tube.
4. Incubate the 20 μl cDNA synthesis reaction was incubated at 25℃ for 5 minutes and then at 42℃ for 1 hour.
5. Inactivate the enzyme at 65°C for 20 minutes. The cDNA product can be directly fed into the qPCR reaction or stored at -20°C.注意事项
Please avoid repeated freeze-thaw cycles.
- 产品数据
生物活性
The nucleic acid glue map shows the effect of M-MLV(H-) reverse transcriptase on total RNA for qPCR detection. The total RNA extracted from HEK293T cells was 2µg. In 20μl reverse transcription system (1µg Total RNA, 120uM Random Hexamer primer, 1X Reaction Buffer, 20U RNase Inhibitor, dNTP Mix (1mM each), After retrotranscription, 1μl of the retrotranscription product was obtained for qPCR amplification and electrophoresis detection of 197bp segment of β-actin cDNA.As shown in the figure, this product has comparable enzyme activity compared with Competitor N's products.
M, marker
Lane 1 Negative Control-1(negative control with no added enzyme only)
Lane 2 Negative Control-2(only negative controls without templates)
Lane 3 UA-M-MLV(H-) Reverse Transcriptase 100U
Lane 4 competing product N 100U
电泳(SDS-PAGE)
1μg (R: reducing condition, N: non-reducing condition).
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