3 U/μl T4 DNA Polymerase, 100 mM K3PO4, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C 10* Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl2, 1000 µg/ml Recombinant Albumin, (pH 7.9 @ 25°C)
操作步骤
Incubate the following reaction at 12°C for 15 minutes
Component
Final Concentration
10X Reaction Buffer
1X
dNTP Mix (10 mM)
100 mM each
T4 DNA Polymerase (3,000 U/ml)
1000 U/ml
DNA
10 ug
Nuclease-free Water
to 10 µl
Total Reaction Volume
10 µl
Stop reaction by adding EDTA to a final concentration of 10 mM and heating to 75°C for 20 minutes
注意事项
Elevated temperatures, excessive amounts of enzyme, failure to supplement with dNTPs or long reaction times will result in recessed ends due to the 3´ → 5´ exonuclease activity of the enzyme.
产品数据
生物活性
The experiment was designed to synthesize two complementary DNA Oligos. Small DNA fragments with 5 'sticky ends were synthesized by annealing, and the sticky ends were completed by T4 DNA Polymerase to form flat ends. 20% Native PAGE glue (which can distinguish the size of 0.1~100bp) was used for electrophoresis, and the enzyme activity was verified. As can be seen from the result, the dsDNA fragment at the sticky end is mended by T4 DNA Polymerase, which has a larger molecular weight, and the electrophoretic band position is above the DNA fragment with the sticky end. Lane 1 Negative Control Lane 2 UA070070-T4 DNA Polymerase 10ng Lane 3 UA070070-T4 DNA Polymerase 20ng Lane 4 UA070070-T4 DNA Polymerase 40ng Lane 5 UA070070-T4 DNA Polymerase 80ng Lane 6 UA070070-T4 DNA Polymerase 0.1ug Lane 7 UA070070-T4 DNA Polymerase 0.2ug Lane 8 UA070070-T4 DNA Polymerase 0.4ug Lane 9 UA070070-T4 DNA Polymerase 0.8ug
大包装询价 
营业执照（三证合一） 
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