Kex2

Kex2 lyophilized
Dissolved buffer: 20mmol/L NaAc-HAc, 2mmol/L Ca2+, pH 5.0~5.5
Reaction buffer:50 mM Tris-HCl、2mM Ca2+、(pH 8.0 @ 25°C)

Kex2 should be reconstituted by the addition of 20–100 μl of Dissolved buffer. Typical reaction conditions are as follows:
Combine 500 ug of sample with reaction buffer *（Recommended 50 mM Tris-HCl、2mM Ca2+、(pH 8.0 @ 25°C))）
Add 5μL(5μg)of Kex2，Incubate at 25°C for 3 hours
Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate Recommended ratio: protease: fusion protein amount ratio 1:20~1:1000

1.Reaction buffer: pH 7.0-9.0, 50mM Tris-HCl, 2mM Ca2+;

2.If the lyophilized powder cannot be used immediately after dissolution, it is recommended to dissolve the lyophilized powder with 20mM (pH5.2) NaAc-HAc, 2mM Ca2+ buffer;

3.The final concentration of the enzyme after dissolution will be stored at -20℃ for storage after dispensing as needed;

4.The optimal reaction pH for Kex2 is pH 9.0, and the optimal pH for storage is 5.0-6.0.