Recombinant Enterokinase (High specific activity)

5 U/μl hEK, 20mM Tris-HCl, 200 mM NaCl, 2mM CaCl2, 50% Glycerol, pH 7.4 @ 25°C

Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:
1、Combine 500 ug of sample with reaction buffer *（Recommended Reaction Buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 (pH 8.0)）
2、Add 1 U of Enterokinase，
3、Incubate at 25°C for 16 hours

Enterokinase is inhibited by high salt concentrations. For optimal activity NaCl concentration should be 50mM or less. The pH of the buffer should be between 6 and 9. The enzyme requires 2 mM Calcium for activity.