Recombinant α-Chymotrypsin, Human

Recombinant α-Chymotrypsin, human lyophilized powder

Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:
Add Recombinant α-Chymotrypsin, human, to a final protease: protein ratio of 1:200 to 1:20(w/w), and incubate sample for 2–18 hours at 25°C. The reaction may be stopped, if desired, by adding 0.5% trifluoroacetic acid.