StartScript® One Step RT-PCR Kit

Component

UA070126-50 Rxns

UA070126-250 Rxns

One-Step Enzyme Mix (25X)

100 μl

5 × 100 μl

One-Step Reaction Mix (2X)

2 × 625 μl

10 × 625 μl

RNase Free dH2O

2 × 1 ml

10 × 1 ml

Component

Volume

One-Step Enzyme Mix (25X)

2 μl

One-Step Reaction Mix (2X)

25 μl

Gene-specific Forward Primer (10 μM)

2 μl

Gene-specific Reverse Primer (10 μM)

2 μl

Template RNA (1 pg~1 μg)

x μl

Rnase Free dH2O

Up to 50 μl

Step

Temperature

Time

Number of Cycles

Reverse Transcription*

48°C

30 minutes

1 cycle

Initial Denaturation

94°C

3 minutes

1 cycle

Denaturation

Annealing**

Extension

94°C

50-65°C

72°C

30 seconds

30 seconds

1 minutes

25–35 cycles

Final Extension

72°C

5 minutes

1 cycle

Soak

4°C

Indefinite

1 cycle