Storage Solution: 50 U/ul T7 RNA Polymerase, 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 10 mM DTT, 50%(v/v) Glycerol, 0.1% (w/v) Triton® X-100 pH 7.9 @ 25°C 10* Reaction Buffer: 400 mM Tris-HCl, 60 mM MgCl2, 10 mM DTT, 20 mM spermidine (pH 7.9 @ 25°C)
操作步骤
1. Assemble the reaction at room temperature in the following order.
Components
Volume
Final Concentration
10X Reaction Buffer
2 µl
1 X
NTP mix, 10 mM each
1 µl
0.5 mM each
RNase Inhibitor（40 U/ul）
0.5 µl
1 U/µl
50 mM DTT
2 µl
5 mM
Template DNA
variable
20 ng–1 µg
T7 RNA Polymerase
1 µl
2.5 U/µl
DEPC-treated Water
Up to 20µl
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2. Incubate at 37°C for 1 hour. For shorter (< 300 nt) transcripts incubate at 37°C for 2–16 hours.
注意事项
Please avoid repeated freeze-thaw cycles.
产品数据
生物活性
The experiment was designed. In a 20µl reaction system (40mM Tris-HCl pH7.9, 2mM Spermidine, 6mM MgCl2, 1mM DTT), PCR products containing T7 Promoter were added as template DNA and incubated at 37℃ for 1h. The reaction was terminated after incubation at 70℃ for 10min, and 10U DNase I was added to digest the template DNA. The RNA transcription product obtained was 541nt. Then 5μl reaction product was removed, 1μl 6X Loading Buffer was added, and 1% agar-gel was used for electrophoresis at room temperature, 1X TAE was used as the electrophoresis solution, 160V for 20min. After electrophoresis, the results were photographed. As shown in the figure, this product has excellent transcription effect. Marker (100~2000 bp) Lane 1 Negative Control (negative control with no added enzyme only); Lane 2 50U UA-T7 RNAP; Lane 3 50U Competitor N-T7 RNAP;
大包装询价 
营业执照（三证合一） 
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