Trypsin，Mass Spectrometry Grade lyophilized 2*Reaction buffer:，100 mM Tris-HCl, 40 mM CaCl2, pH 8.0 @ 25°C
操作步骤
1 Trypsin, Mass Spectrometry Grade should be reconstituted by the addition of 20–200 μl of 2 mM HCl 2 Set-up a typical reaction as follows 1）Add the following components in sequence
Components
Volume
Substrate protein
5 μL (about 2 ug)
ddH2O
4.5 μL
2*Reaction buffer
10 μL
100 ng/μL Tyrpsin
0.5 μL
2）Incubate at 37°C for 2~4 h
注意事项
1. Substrate must be in phosphate-free buffer to prevent calcium precipitation with both reconstituted enzyme and enzyme buffer.
2. Storage buffer: 2 mM HCl, 2 mM CaCl2.
产品数据
生物活性
In terms of sequence coverage, UA070130 demonstrates a slight advantage, with enzyme P and enzyme Y exhibiting coverage values that are 1.3% and 2.3% lower, respectively. However, when considering peptides with no more than three missed cleavage sites, a different trend emerges. Enzyme Y yields the highest proportion, followed by enzymes UA070130 and P, which show marginally lower values. These results indicate that the three enzymes display comparable efficacy in protein digestion.
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营业执照（三证合一） 
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