The EGFR[T790M/C797S/L858R] activity was detected using HTRF technology. The reaction was performed by incubating the EGFR[T790M/C797S/L858R] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
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