The EGFR[T790M/C797S] activity was detected using HTRF technology. The reaction was performed by incubating the EGFR[T790M/C797S] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
电泳(SDS-PAGE)
M MW Standard 1 2 μg EGFR[T790M/C797S] 2 1 μg EGFR[T790M/C797S]
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