This Week's Special Recommendation: DNase I

DNase I

DNase I, or Deoxyribonuclease I, is an endonuclease capable of digesting both single-stranded and double-stranded DNA, producing deoxyribonucleotides or single-stranded or double-stranded oligodeoxyribonucleotides. The hydrolysis products of DNase I have a 5' phosphate group and a 3' hydroxyl group. DNase I activity is calcium-dependent and can be activated by magnesium or divalent manganese ions. In the presence of magnesium ions, DNase I randomly cleaves double-stranded DNA at any site. In the presence of divalent manganese ions, DNase I cleaves both strands of DNA at the same site, generating blunt ends or sticky ends with 1-2 nucleotide overhangs.

Catalog Number: UA070036

Name: DNase I

Specifications: 1 KU / 5 KU

Enzyme Activity Definition: One unit of activity is defined as the amount of enzyme required to completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C.

The results of 1μg pBR322 plasmid digestion separated under different quantity of DnaseⅠ, The reaction was incubated for 10 minutes at 37°C, and 1% agarose gel was used for electrophoresis analysis after reaction.

M, marker;

Lane 1 1μg pBR322;

Lane 2 1μg pBR322 add 4U DNase I

Lane 3 1μg pBR322 add 2U DNase I

Lane 4 1μg pBR322 add 1U DNase I

Lane 5 1μg pBR322 add 0.5U DNase I

Lane 6 1μg pBR322 add 0.25U DNase I

Lane 7 1μg pBR322 add 0.125U DNase I

Applications of DNase I

1. DNA Removal During RNA Extraction

2. Preparation of DNA-Free Templates for Reverse Transcription and Degradation of DNA Templates Post-Transcription

3. Digestion of DNA Probes During rRNA Removal

4. Footprinting Analysis of DNA-Protein Interactions

5. Positive Control for DNA Cleavage in TUNEL Assays for Apoptosis Detection

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