Precision interaction, one "probe" reveals all

Precision Interaction, Unveiled at a Glance

TR-FRET Technology Empowers PROTAC/Molecular Glue Research

Kit Highlights:

• Ready-to-use kit, no optimization required, direct detection saves time
• Homogeneous assay, wash-free, simplified workflow, compatible with high-throughput
• High sensitivity, low background, TR-FRET technology ensures stable and reliable signals
• Multiple specifications available: 500T, 2500T, 10000T
• Customization accepted

1. Binding Screening and Detection---Competitive Binding Assay

Human CRBN Ligand Screening Assay Kit

Detection Principle:

This kit efficiently and accurately screens compounds or ligands that specifically bind to CRBN, providing the inhibition constant Ki, offering a powerful tool for the design and optimization of PROTAC molecules.

Binding Activity and Inhibitor Validation:

Validation of Thalidomide-Ac binding to CRBN/DDB1. Results show that when protein concentration remains constant, the detection signal increases with higher Thalidomide-Ac concentrations. Lenalidomide, Pomalidomide, and PROTAC BET Degrader-1 can compete with Thalidomide-Ac for binding to CRBN/DDB1.

2. Binding Screening and Detection---Complex Formation Assay

These products are primarily used for studying the formation of PROTAC (Proteolysis Targeting Chimera)-mediated ternary complexes.

3. Target Protein Degradation Detection